Keratoconus is not associated with mutations in COL8A1 and COL8A2.

Purpose:To evaluate the suggested role of the COL8A1 and COL8A2 genes in the pathogenesis of the corneal ectatic disorders keratoconus and keratoglobus through mutation screening in affected patients.Methods:DNA extraction, polymerase chain reaction amplification, and sequencing of COL8A1 and COL8A2