Ciclo de vida de Meloidogyne incognita (KOFOID Y WHITE) Chitwood en dos cultivares de Phaseolus vulgaris L.

The experiment was conducted under semi-controlled conditions from January to March 2017 with the objective of determining the duration of the life cycle of Meloidogyne incognita (Kofoid y White) Chitwood and the egg production per adult females on plants of the common beans (Phaseolus vulgaris L.) cultivars "BAT-306" and "Triunfo- 70". The pots with the plants inoculated with 2000 J2 from a pure population of M. incognita were maintained at 28oC in isolators at CENSA and irrigated every other day. For the analysis of the life cycle, the roots were washed with tap water, examined externally, and the swollen areas or with well-defined galls were opened to count the different larval stages and mature states. Once the first mature females appeared, the evaluation was continued until 96 hours after the appearance of females with eggs. From the samples of plants with roots that contained eggs, 20 females with their eggs were taken at random daily, and the number of eggs x female-1 was counted. The presence of J2 on the plants of both cultivars started being detected 24 hours after inoculation. "BAT-306" resulted a susceptible cultivar, and M. incognita cycle was completed in 28-29 days, while the cultivar "Triunfo-70" was resistant and the nematode did not complete its biological cycle.