Modification of Human Fibroblast Properties in Microtransplant

We studied the properties of human skin fi broblast in fi lamentous polyglycolic microtransplant. Fibroblast adhesion to the microtransplant fi laments is followed by the formation of a network cross-linked with fi broblasts. The cells rapidly proliferate during the fi rst few days; after transfer of the microtransplant to the standard culture fl ask, the cells migrate to the plastic and continue proliferation. The cells are uniform and exhibit high colony-formation capacity. The bundles of microtransplant fi laments persist in the culture for several days and through the cells completely leave them, the area around these fi laments remains the most populated for 40 days. Mitotic cells are seen in the immediate proximity to the degrading fi laments of the transplant. The effect of cell “rejuvenation” in the microtransplant can be explained by selection of cells by their adhesion to relatively thin (about 15 μ) fi laments, which excludes large old cells.