The effect and mechanism of miR-451 on migration of glioma cell line U251

Objective To investigate the effect of microRNA-451 (miR-451) on migration and its potential molecular mechanism in glioma cell line U251.Method The expression of miR-451 in glioma tissues and brain tissues was identified by qRT-PCR.U251 cells were divided into two groups,miR-451 inhibitor group and control group,for in vitro experiments.Oligonucleotide of miR-451 inhibitor was transfected into human glionblastoma cell line U251.The migration of each group cells was examined by Wound Healing assay and Transwell assay,and the expression of protein of GTP-Rac1 and Rac1 were determined by Western blot respectively.Results The levels of miR-451 expression in glioma tissues were significant lower than brain tissues (P ＜0.01).Wound Healing assay and Transwell assay discovered that the migration of miR-451 inhibitor group cells was obviously increased compared with control group(P ＜ 0.01).Western blot demonstrated that the expression of GTP-Rac1 was up-regulated in miR-451 inhibitor group cells(P ＜0.01) and no significantly different was found in the expression of Rac1of each group (P ＞ 0.05).Conclusions miR-451 inhibits U251 cells migration through reducing activation of Rac1. Key words: Glioma;  miR-451 ;  Migration;  Invasion