Comparison on clinicopathological features, genetic instability, TP53 and KRAS gene mutations between well and moderately differentiated colorectal adenocarcinoma.

Objective: This study is to evaluate the differences of clinicopathological and genetic features between well and moderately differentiated colorectal adenocarcinoma.Methods: A total of 1073 colorectal carcinoma cases were retrospectively reviewed to show the ratio of well and moderately differentiated adenocarcinoma in a timely fashion.The clinicopathological features of 328 well or moderately differentiated adenocarcinoma cases were evaluated.In 129 cases,Laser Scanning Cytomertery was used to assess DNA ploidy,the microsatellite instability status were studied by High Resolution Fluorescent Microsatellite Analysis assay.Mutation hotspots of TP53 and KRAS genes were directly sequenced.Results: The ratio of moderate differentiated adenocarcinoma in all pathology types was increased in the recent decade.Comparing with well differentiated adenocarcinoma,the moderately differentiated carcinoma showed more invasive characteristics,such as late stage,deeper infiltration,higher lymphatic metastatic rate,stronger vein invasion (P0.05).The genetic instability patterns reflected by DNA aneuploidy or microsatellite instability of the well and moderately differentiated carcinoma were similar.The mutation rates of TP53 and KRAS genes were similar.However,frameshift mutation of TP53 was detected only in well differentiated adenocarcinoma;the frequency of trasversion mutations of KRAS were significantly higher in moderately differentiated adenocarcinoma.Conclusions:TRAIL and docetaxel have synergistric killing effects on nasopharyngeal carcinoma CNE2 cells,which has a promising prospect in the treatment of nasopharyngeal carcinoma.