P05-10. Sequential immunization with a subtype B HIV-1 envelope quasispecies elicits broader neutralization than vaccination with a single envelope clone

Background HIV-1 evolves rapidly within the host, resulting in the development of diverse HIV-1 variants called a viral ''quasispecies'' population. Envelope (Env) is the only target of neutralizing antibodies (NAbs), which can prevent infection of target cells. NAbs develop over time as the Envelope diverges. A major goal of HIV-1 vaccine efforts, so far elusive, is the design of Env-based immunogens effective at eliciting broad NAbs. We hypothesized that programming the B cell response could be achieved by exposing the host to a collection of env variants representing the viral quasispecies members.