苄嘧磺隆降解菌BP-H-01的分离及降解特性

[Objective] The study was done to isolate the effective fungi strains for biodegrading bensulfuron-methyl, one of the most widely used herbicides in China, and provide prospect candidate biological preparations for reducing sulfonylurea herbicide residues in the soil. [Method] The degrading fungi were isolated in Czapek media from poor nutrient or serious contaminated soil collected from several provinces in China, and then screened in the media of bensulfuron-methyl as only carbohydrate. The effective degrading fungi were determined according to the degrading rate and the morphology and the degradation characteristics were preliminarily identified. [Result] A bensulfuron-methyl highly effective degradation fungus strain BP-H-01 with above 80% of relative degradation rate to 25-500 mg/L of bensulfuron-methyl, which was screened from 78 fungi and bacteria strains, tolerated 500 mg/L of bensulfuron-methyl. Based on morphological study, BP-H-01 was identified as Aspergillus sp. The optimum conditions for degrading Bensulfuron-methyl were pH 7.5, 28℃ and 2 g/L of the inoculated quantity respectively. In this condition, the degradation rate achieved 84.5%. [Conclution] Fungus strain BP-H-01 can biodegrade remarkably bensulfuron-methyl specially.