Isolation, identification and characterization of f eather degrading bacteria

The present study deals with isolation, identificat ion and characterization of feather degrading bacte rium. The keratinolytic bacteria were isolated from feather d umped soil. The colonies showed higher keratina se production was identified as Bacillius sp, as per Bergey’s manual method. Keratinase producing Bacillus s p, showed higher enzymes production. The crude filtrate showed specific activity of (>2.825)IU/mg and the protein of 4 mg/ml. Bacillus sp, produced keratinase at th e pH 7 ‐ 7.5 at the temperature of 40°C 2% fe ather meal as a substrate and incubation time of 96 hrs respe ctively which indicated that the crude keratinase enzyme produce by Bacillus sp was classified as an alk aline protease. The protein profile of Bacillus sp was analyzed in SDS ‐ PAGE. It showed that the presence of sin gle band which corresponds to keratinase acti vity. FTIR Fourier Transform Infra red Spectroscopy showed that the change in the functional group was catalyzed by unique enzymes of Bacillus sp. The keratin degra ding gene sequence of isolated Bacillus sp, was analysed further studies.