Microbore high‐performance liquid chromatography mass spectrometry

The use of microbore liquid chromatography columns for high-performance liquid chromatography/mass spectrometry provides improved sensitivity for interfaces of the direct liquid introduction and moving belt types. In the latter case the use of the technique also assists in the handling of mobile phases containing a high percentage of water. Methods for improving the efficiency of microbore columns made from PTFE are described together with the use of a manual column switching technique to assist in some of the problems encountered due to column overload with microbore liquid chromatography columns. The utility of microbore liquid chromatography/mass spectrometry is illustrated by the analysis of an aqueous solution of phenolic compounds.