Effets antiprolifératifs et proapoptotiques du bisphénol A dans les cellules trophoblastiques humaines JEG-3

Summary Different studies performed in rodents revealed that Bisphenol-A (BPA), an environmental compound, altered early embryonic development. However, little is known concerning the direct effects of BPA on human implantation process. Thus, we decided to study in vitro BPA's effects on proliferative capacities of the human trophoblastic cell line, JEG-3. For this purpose, we first have shown that JEG-3 cells express the specific BPA receptor, namely estrogen-related receptor γ1 (ERRγ1). Secondly, once the non-toxic effect of BPA on JEG-3 cell viability was verified, [ 3 H]-thymidine incorporation experiments were performed and revealed that BPA significantly reduced cell proliferation. The results also showed that BPA induced JEG-3 apoptosis as reflected by DNA fragmentation experiments. In conclusion, we describe here the direct impact of BPA on trophoblastic cell number mediated through both antiproliferative and proapoptotic effects and could then participate in the impact of BPA on placental development.