An Evaluation of the Intrinsic IgG Production Capabilities of Different Chinese Hamster Ovary Parental Cell Lines

To better characterize differences in expression and secretion capacity, we quantitatively analyzed production of Green Fluorescent Protein (GFP) and secretion of recombinant human IgG in transiently transfected CHOK1SV, ECACC K1 and CHO DG44 parental cell lines. By analyzing these production trends in a transient transfection system, we were able to compare recombinant protein production and secretion between the different CHO parental cell lines independent of integration site effect.