The Proliferation of Two Bladder Tumor Cell Lines Inhibited by Four Cytotoxins from Naja Atra in Vitro

Objective The inhibiting tumor cell proliferate actives of four cytotoxins(CTX-10,CTX-11,CTX-12 and CTX-13) were tested using tow human bladder tumor cell lines(BIU-87 and EJ).Methods The inhibiting tumor cell proliferate actives of four cytotoxins were tested by crystal violet colorimetric analysis.Results All of the four CTXs showed favorable inhibiting bladder tumor cell proliferate actives and the inhibitory effects showed a definite dose-effect relationship.The IC_(50) of CTX-10,CTX-11,CTX-12 and CTX-13 on BIU-87 were 6.03,6.27,2.25 and 2.89mg/L,the IC_(50) on EJ was 8.02,7.89,3.05 and 2.76mg/L respectively.Conclusion Four CTXs owned favorable inhibiting bladder tumor cell proliferate actives.They are largely potential anticancer drug for bladder carcinoma.