Expression of CD14 and CD15 in HL-60 Cell Affected by 190-subunit Cytoplasmic Domain of Leukemia Inhibitory Factor Receptor

To investigate the effect of gp190-subunit of leukemia inhibitory factor(LIF) receptor on the expression of CD14 and CD15 in HL-60 cell and the mechanism of leukemia cell proliferation and differentiation disorder,the gp130/190 chimerical receptor was constructed by exchanging the cytoplasmic domain of gp130 to gp190,with 190 C-terminal polypeptide(190CT) being constructed by PCR.The expressed products in HL-60 were measured by immunohistochemistry and flow cytometric analysis.Compared with the group of pcDNA3.0,higher level of CD15 expression was found in the group of 130/190(P0.01),while lower level of CD15 expression in the group of 190CT(P0.05).However,there was a little expression for CD14 in HL-60 cells and expression level was nearly the same between the two groups.The results suggested that LIF promotes the differentiation of HL-60 cells with LIFα subunit toward granulocyte instead of monocyte.The effect was mediated by the cytoplasmic domain of gp190.190CT could interfere with the signal transduction of LIFα subunit.