Investigation of characteristics of effluent DON derived from conventional activated sludge (CAS) and predenitrification biological removal (BNR): In terms of proteins and humic substances.

Abstract Upgrading wastewater treatment plants (WWTPs) from conventional activated sludge (CAS) to predenitrification biological nutrient removal (BNR) results in improved removal of dissolved inorganic nitrogen (DIN) from wastewater. However, changes in dissolved organic nitrogen (DON) with these WWTP upgrades and their potential impacts on receiving waters have been little researched. In this study, we investigated characteristics of effluent DON derived from CAS and predenitrification BNR, paying special attention to proteins and humic substances. Through a lab-scale reactor study and analysis of full-scale WWTP effluents, we found that in predenitrification BNR effluent, proteins are much more dominant than humic substances, whereas in CAS effluent, proteins and humic substances are similarly abundant. In terms of molecular weight, the majority of proteins were present in the effluent’s low molecular weight (LMW) fraction (