Changes of composition and relative contents of fatty acid in myocardium of Kunming mice infected with Trichinella spiralis.

To explore the mechanism of myocarditis caused by Trichinella spiralis and host resistance against T.spiralis,myocardium samples from the Kunming mice infected with T.spiralis on days 5,7 and 35 post-infection,respectively and a sample from the healthy mice were collected.The total lipid were extracted from the samples by AIB method,transestered with 14% BCl3-methanol and analyzed by GC-MS.The main fatty acid contents of the infected myocardium samples were significantly different from that of the normal myocardium(P0.05).The content of the polyunsaturated fatty acids and the total n-6 fatty acids of the infected myocardium samples decreased significantly because the content of linoleic acids decreased significantly from 17.23%±2.10% to 9.21%±1.25%(P0.05).The contents of saturated fatty acids of the infected myocardium were higher than that of the normal myocardium,mainly due to increase of the contents of palmitic acid and stearic acid.In the infected myocardium samples,with prolongation of the time of infection,the contents of C18:2 and C20:4 decreased first,and then returned to normal levels,and the levels of C22:5 and C22:6 increased first,and then reduced to normal levels.