Novel Injection Method in Preparative Supercritical Fluid Chromatography

Components are listed in the figure legend. The DLI method dilutes the sample solvent with the mobile phase fluid when the sample is merged with the solvent of the bypass line. The bypass line also prevents the pressure surge when the injector valve is turned. The SF-NAV software managed the total system control. Determination of dilution ratio in the DLI system It is important to determine the dilution ratio when the injected sample is merged with the mobile phase fluid (a mixture of CO2 and modifier solvent) in the bypass line.