Protein Conservation Method Affects MamC-MediatedBiomineralization of Magnetic Nanoparticles

Magnetite nanoparticles in the magnetosomes formed by magnetotactic bacteria present unique magnetic properties that make them the ideal nanoparticle with potential use in several biotechnological applications. These magnetoliposomes are organelles formed by crystals of magnetite (Fe3O4) or greigite (Fe3S4) surrounded by a lipid bilayer. However, scaling up the production of these nanoparticles cannot be achieved currently because of the slow growth and the strict physiological characteristics of the magnetotactic bacteria. An alternative to solve this problem is the biomimetic approach, which involves the in vitro production of magnetite nanoparticles mediated by magnetosome membrane proteins, expressed as recombinant. MamC-mediated magnetite nanoparticles (BMNPs) have been recently proposed as some of the best biomimetic (magnetosome-like) nanoparticles for potential use in targeted drug delivery and hyperthermia treatments. However, the production of these crystals still needs to be scaled up. One of t...