The initial exploration of effect of K562-derived microvesicles on their parental cells

Objective:To investigate the effect of microvesicles(MVs)derived from K562 cells on the proliferation and apoptosis of their parental cell and analyze relevant mechanism.Method:K562-MVs were extracted from cell culture supernanant and labeled with a PKH26 red fluorescent labeling kit.Human umbilical vein endothelial cells(HUVEC)were incubated with the PKH26-labeled K562-MVs for 10 h and subjected to fluorescence microscope and photographed.K562 cells were incubated with different concentrations of K562-MVs for 48 h.Cell proliferation was assayed with CCK8 kit and cell apoptosis was analyzed with Flow cytometry.Expression of bcl-2 was examined by semi-quantitative RT-PCR.Result:K562-MVs could integrated with HUVEC cells.And K562-MVs could promote proliferation of K562 cell with the concentration of more than 60 μg/ml.The proliferation rate was concentration-dependent,and besides,differences of proliferation rates between each group were statistically significant.Compare to the control group,apoptosis rate decreased and expression level of bcl-2 increased in the 120 μg/ml group(P0.05).Conclusion:K562-MVs are not meaningless cell debris,but the information carrier with bioactive molecules.K562-MVs can promote their parental cells proliferation and inhibit their apoptosis.K562-MVs may play its anti-apoptotic effect via increasing expression level of bcl-2.