Desensitization using Self-Antigen dsDNA inhibits B and T cell Functions by Modulating T-Reg cell as Regulator Immune System in Lupus Mice Model

The aim of this study was to develop a novel therapeutic method for improving immune system regulation in SLE using self-antigen dsDNA . A total of 25 female BALB / c mice were divided into 2 groups: 20 mice received a single i ntraperioneal injection of 0.5 mL pristane for lupus induction and 5 mice as healthy controls. Starting at 8 weeks after injection, 15 pristane-induced lupus mice were divided into three groups: 0,005 µg, 0,05 µg, and 0,5 µg. The doses would increase ten times every week. dsDNA were complexed with the cationic polyethylenimine before injection, 25 mice were analyzed for autoantibodies levels (dsDNA, ANA) and TGF-β from serum using ELISA and T-Reg, dendritic cell maturation from spleen using flowcytometry. Escalating dose antigen spesific therapy with dsDNA decreased ANA levels ( 21.6 vs 30. 4 p=0.02) significantly compare to positive control , decrease d anti-dsDNA (20.7 vs 68.3 p=0.08), decrease d dendritic cell maturation (1.96 vs 2.94 p=0.84) and decre a se s Th17 cells (3.62 vs 5.62 p=0.18). D e sensitization using self-antigen dsDNA was increased T-reg proliferation (10.57 vs 18.38 p=0.019) and level of TGF-β (732 vs 261.02 p=0.03) significantly compare to control positive. In conclusion, d e sensitization using self-antigen dsDNA was able to modulate T-Reg as a regulator immune respon and inhibit B and T cell functions in lupus mice model.