Efeito dos extratos de jabuticaba e pequi na expressão gênica de enzimas antioxidantes em células musculares C2C12 de camundongos

Jaboticaba and pequi fruits contain bioactive compounds, such as polyphenols, which present antioxidant actions; however, the molecular mechanisms by which these effects are achieved are not fully elucidated. In the present study, mouse muscle cells (C2C12), induced or not by oxidative stress with hydrogen peroxide (H2O2), were treated with jaboticaba peel extract (JPE) and pequi aqueous extract (PAE) in order to evaluate their influence on expression of the following antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), glutathione s-transferase (GST) and glutathione peroxidase 1 (GPX1). Treatments with JPE (150 µg gallic acid equivalents mL-1 extract) and PAE (30 µg GAE mL-1 extract) increased CAT and GST mRNAs expression in muscle cells. In cells induced by oxidative stress with addition of H2O2, peroxide detoxification action attributed to the GST enzyme was verified. The extracts beneficial effects may be due to the synergistic activities of their various phenolic compounds and other constituents.