A rapid and sensitive immunoassay for antibodies against alloantigens on human platelet glycoproteins (BIPA)

Abstract A rapid and sensitive enzyme immunoassay has been developed for the detection and specification of alloantibodies against human platelet antigens (HPA) on platelet membrane glycoproteins. Human platelet glycoprotein complexes were coupled by monoclonal antibodies to immunomagnetic beads with sheep anti-mouse IgG directed antibodies. These beads served as solid-phase target antigens for human alloantibodies in a modified MAIPA assay. This test provided high sensitivity, permitting the identification of antibodies against membrane receptors and required only 1 h of assay time. The specificity and sensitivity of this assay were evaluated in serum samples from polytransfused patients and compared with standard immunoassays employing antigen coated immunowells.