The influence of different peripheral venous blood sampling methods on hemolysis rate in emergency room

Objective To compare the influence of three different peripheral venous blood sampling methods on hemolysis rate.Methods A total of 1036 patients who needed both intravenous infusion and venous blood sampling were randomized to three groups receiving different blood sampling methods:obtaining the blood samples through the intravenous catheter connected with the vacutainer(group A,n=343),obtaining the blood samples through the intravenous catheter connected with the syringe(group B,n=365),and obtaining the blood samples through the vacutainer system(group C,n=328).The rates and degree of hemolysis was recorded.Results The hemolysis rates among the three groups were significantly different(P0.05),which were 14.72 percent,25.21 percent,and 3.50 percent in group A,B,and C,respectively.The blood in the procoagulant inert separation tube(yellow cap) was more likely to hemolysis than that in the sodium citrate coagulation test tube(blue cap).The hemolysis rate of 18G,20G,and 22G intravenous catheters was 9.32 percent,19.81 percent,and 32.58 percent,respectively.Conclusions The hemolysis rate of blood obtained though the vacutainer system is the lowest,and the hemolysis rate of blood obtained though the intravenous catheter connected with the syringe is the highest.