High-Throughput Implementation of the NanoBRET Target Engagement Intracellular Kinase Assay to Reveal Differential Compound Engagement by SIK2/3 Isoforms:

Hyun Yong Jin,Yanyan Tudor,Kaylee Choi,Zhifei Shao,Brian A. Sparling,Joseph G. McGivern,Antony Symons

High-Throughput Implementation of the NanoBRET Target Engagement Intracellular Kinase Assay to Reveal Differential Compound Engagement by SIK2/3 Isoforms:

2019

Hyun Yong Jin
Yanyan Tudor
Kaylee Choi
Zhifei Shao
Brian A. Sparling
Joseph G. McGivern
Antony Symons

The real-time quantification of target engagement (TE) by small-molecule ligands in living cells remains technically challenging. Systematic quantification of such interactions in a high-throughput...

Keywords:

Kinase
Cell biology
Intracellular
Gene isoform
Chemistry
Ligand
target engagement

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