Chemical constituents from dried sorophore of cultured Cordyceps militaris

Objective:To investigate the chemical constituents of the dried sorophore of cultured Cordyceps militaris.Method: Compounds were isolated and purified by macroporous adsorption resin and silica gel column chromatography.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data(IR,FAB-MS,1H-NMR and 13C-NMR).Result: Nine compounds were isolated and identified as: ergosta-4,6,8(14)-tetraen-3-one(1),citrostadienol(2),tetracosanoic acid 2,3-dihydroxypropyl ester(3),ergosterol(4),ergosterol peroxide(5),ergosta-7,22-dien-3β,5α,6β-triol(6),cordycepin(7),adenosine(8),N-(2-hydroxyethyl) adenosine(9),respectively.Conclusion: Compounds 1-3,6,9 were separated from the sorophore of cultured C.militaris for the first time.