Molecular epidemiology of highly pathogenic porcine reproductive and respiratory syndrome virus in China.

2009 
In the summer of 2006,a large-scale outbreak of highly pathogenic PRRS in China was reported.To fully understand the knowledge of highly pathogenic PRRSV epidemiology and the extent of genetic diversity in China,we analysed 474 samples obtained from 19 provinces using RT-PCR assay.294 samples of them were positive.Subsequent sequencing of the PCR products from Nsp2 and ORF5 and phylogenetic analysis revealed that all positive strains are highly homologous to each other,may have evolved from the same ancestor.Moreover,they were most similar to HB-1.At the same time,we observed a unique molecular hallmark in these viral isolates,a discontinuous deletion of 30 amino acids in nonstructural protein 2(Nsp2).
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