감나무(Diospyros kaki Thunb)잎 추출물에 대한 화장품약리활성 검증

In this study, we investigated the bioactive characteristics of persimmon (Diospyros kaki Thunb) leaf extracts possibly to utilize as cosmeceutical ingredients. The bioactive characteristics of cosmeceutical ingredients in persimmon leaves including anti-oxidation activity, whitening effect and anti-wrinkle effect were examined. The extraction yields by using hot water and ethanol were 18.71% and 12.87%, respectively. For anti-oxidation experiments, the electron donating activities of both hot water- and ethanol-extracts were very high showing over 62.4% and 71.5% at 50 ppm, respectively, and increased in a concentration-dependant manner. In the measurement of SOD-like activity, ethanol- and hot waterextracts showed 63.7% and 58.5% at 1,000 ppm, respectively. In the inhibitory activity of xanthine oxidase, the ethanol extract was significantly higher (85.2%) than hot water extract (68.7%) at 1,000 ppm. In inhibitory activity of tyrosinase, no inhibitory activity was detected at the concentration of 10 ppm, however it showed an inhibitory activity of 68.7% at the concentration of 50 ppm. Both collagenase and elastase inhibitory activities were higher in the ethanol extract compared to that of hot water extract. At the concentration of 50 ppm, the collagenase inhibitory activity of ethanol extract was over 60%. Taken together, these data suggest that the persimmon leaf extract is effective in whitening and anti-wrinkling, thus it might strongly be considered as potential functional cosmetic components.