12 C 6+ 离子束与电子束辐照对薰衣草当代诱变效应的比较
2014
采用100 MeV 的12C6+ 离子束和1.2 MeV 的电子束辐照薰衣草干种子,研究了2 个品种的薰衣草干种子对不同辐射源的辐照生物学效应,以期找到薰衣草干种子的最佳诱变参数。结果表明:不论是薰衣草701 还是702,经12C6+ 离子辐照后,其发芽率表现为先增大后减小的趋势;经电子束辐照后,发芽率随着剂量的升高而降低,电子束辐照后的发芽率要低于12C6+ 离子束。另外,2 个品种的薰衣草经12C6+ 离子辐照后,其胚轴胚根长度以及幼苗鲜重也较电子束辐照后的大。由此可知,重离子的辐照效果优于电子束的,有利于后期筛选出新的薰衣草突变株。 To determine the optimal mutagenic parameters to Lavendula, the biological effects on the development of dry seeds of two Lavendula varieties (701 and 702) were comparatively studied between 100 MeV 12C6+ ion and 1.2MeV electron beam. The results showed that the germination percentage of 701 and 702 increased firstly and then decreased with the dose increased by using carbon ion irradiation. However, the germination percentage of 701 and 702 decreased with the increment of radiation dosage after being irradiated by electron beam. Furthermore, the results also showed that the germination percentage of Lavendula irradiated by electron beam was lower than that of carbon ion irradiation. In addition, the length of hypocotyl, embryonic root and fresh weight of seedlings of 701 and 702 irradiated by carbon ions were superior to those of electron beam irradiation. In brief, carbon ions are moresuitable for the mutation breeding of Lavendula compared to electron beams, which would contribute to obtain more novel mutants in the future.
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