Induction of hepatic CYP1A in channel catfish increases binding of 2-aminoanthracene to DNA in vitro and in vivo

Data are presented from in vitro and in vivo studies that indicate cytochrome P4501A (CYP1A) in channel catfish (Ictalurus punctatus) hepatic tissue activates 2-aminoanthracene (AA) to a reactive metabolite that binds to DNA. Channel catfish were injected i.p. with vehicle or 10 mg/kg b-naphthoflavone ( bNF) on two consecutive days. Two days after the final injection of vehicle or bNF, vehicle or [ 3 H]AA was injected i.p. at 10 mg/kg, creating four different treatments: vehicle only, bNF only, [ 3 H]AA only, and bNF/[ 3 H]AA. Hepatic tissue was examined for CYP1A