Confirmation of the anomeric structure of galacturonic acid in the galacturonosyl-ceramide of Sphingomonas yanoikuyae.
2006
The anomeric structure of glycosphingolipids significantly influences their activity to stimulate natural killer T cells. In this study the chemical structure of the galacturonosyl-ceramide in Sphingomonas yanoikuyae, designated GSL-1′sy, was re-examined to prove the anomeric structure of the D-galacturonic acid (GalA) in the lipid, which was reported as β-configuration by Naka et al., but was suggested as α-configuration in our preliminary study. GSL-1′sy was purified from the bacterial cells with the same procedure as Naka et al. The 1H-NMR analysis of GSL-1′sy revealed that the coupling constant of the anomeric proton of GalA was 3.0 Hz, indicating that GalA in GSL-1′sy is α-anomer, the configuration active for the stimulation of natural killer T cells.
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