Quantification of specific E. coli in gut mucosa from Crohn's disease patients.
2011
Abstract We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli . The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
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