Streptavidin binding as a model to characterize thiol–ene chemistry-based polyamine surfaces for reversible photonic protein biosensing

Biotin- and iminobiotin-bonded surfaces obtained by thiol–ene chemistry and subsequent modification with polyamines were characterized with respect to streptavidin-binding capacity and reversibility for photonic biosensing using X-ray photoelectron spectroscopy and Mach–Zehnder-interferometric sensors. The streptavidin–iminobiotin system was exploited for reversible multilayer deposition and determination of affinity constants on each layer.