Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′-hydroxyl acylation of RNA

An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA–RNA mixture, a selective 2′-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.