Method of identifying protein phosphorylation modification sites on the basis of tandem mass spectrometry

The invention provides a method of identifying protein phosphorylation modification sites on the basis of tandem mass spectrometry. According to the method, application result data is searched by tandem mass spectrometry MGF-formatted (mascot generic file-formatted) data and mascot and pFind databases; programming is performed by a Perl language program; identified protein phosphorylation modification sites are re-located fast; and the re-located protein phosphorylation modification sites are calculated by a new scoring algorithm. The method is more creditable and also allows for fast automatic batch exporting of mass spectrograms related to the protein phosphorylation modification sites. The method has the advantages that operation is simple, locating information of the modification sites is highly creditable and the extracted mass spectrograms are high in resolution. The method is well applicable to the field of proteomics study.