Cytotoxic and anti-angiogenic effects of lanostane triterpenoids from Ganoderma lucidum

Abstract Two new lanostane triterpenes, 3 α ,12 β ,15 α -triacetoxy-5 α -lanosta-7,9(11),24-trien-26-oic acid ( 1 ) and 5 α -lanosta-8,24-diene-26,27-dihydroxy-3,7-dione ( 2 ), together with sixteen known compounds ( 3 – 18 ) were isolated from the fruiting bodies of the Vietnamese mushroom Ganoderma lucidum. Their chemical structures were determined by extensive spectroscopic (IR, HR-EI-MS, 1D and 2D NMR) analyses. Potential cytotoxic activities of these compounds were evaluated against human non-small cell lung adenocarcinoma (A549), breast adenocarcinoma (MCF-7), and prostatic small cell carcinoma (PC-3). Among the compounds, 3 α ,12 β ,15 α -triacetoxy-5 α -lanosta-7,9(11),24-trien-26-oic acid ( 1 ) showed significant cytotoxic activity against PC-3 cells with an IC 50 of 11.5 μM. In studies of anti-angiogenesis activity, ganoderic acid F ( 17 ) was found to have the most potent inhibitory effect on the formation of capillary-like structures of human umbilical vein endothelial cells.