Application of ultrasound in stabilizing of Antarctic krill oil by modified chickpea protein isolate and ginseng saponin

Abstract Mixed protein-surfactant systems are widely used for stabilizing emulsions in the food industry. This study investigated the effects of ultrasound pretreatment on the structural and functional properties of chickpea protein isolate (CPI) and its binding interaction with ginseng saponin (GS). Ultrasound pretreatment induced the unfolding of CPI and promoted the binding affinity of CPI to GS, which was confirmed by fluorescence spectroscopy. Circular dichroism spectra demonstrated that the addition of GS increased the α-helix content by 11.20% and reduced the β-sheet, β-turn and random coil content by 3.70%, 1.30% and 6.00%, respectively. Compared with the U0-GS complex, the U15-GS complex stabilized emulsions showed higher (P