Induction of chemiluminescence in human polymorphonuclear leukocytes by the calcium ionophore A23187

1978 
Human polymorphonuclear leukocytes (PMNL) exhibit a characteristic burst of oxidative metabolism following activation by suitable particles [ 11. This burst is associated with the formation of several potentially bactericidal metabolites of molecular oxygen including superoxide anion, hydrogen peroxide and hydroxyl radical. Phagocytosing PMNL also generate electronically excited state(s), as manifested by the phenomenon of chemiluminescence (CL) [2]. It was proposed that relaxation of singlet oxygen (to its triplet ground state) and/or relaxation of excited carbonyl moieties formed during the respiratory burst may provide a basis for this phenomenon. The formation of singlet oxygen via the myeloperoxidase (MPO)-hydrogen peroxide-halide reaction has been demonstrated [3], which is operative in phagocytosing PMNL. Moreover, analysis of the CL spectra for the MPO-HzOz-halide and PMNL reac
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