Creatine supplementation upregulates excitation-contraction coupling in C2C12 myotubes

The main goal of this work was to investigate whether creatine (Cr) might be able to regulate skeletal muscle excitation-contraction (EC) coupling. Myotubes from a C2C12 cell line were exposed to Cr (25 mM, 2-4 hours). Subsequently, the activity of L-type Ca 2+ channels and voltage-gated Ca 2+ release (VGCR) were investigated using the whole-cell patch-clamp technique.