The Relationship Between the +491 C＞T Polymorphism of β2-adrenergic Receptor Gene and Lipid Level in Hazakh of Xinjiang

Objective To investigate the distribution of β2-AR+491C＞T polymorphism in Hazakh of Xinjiang and the relationships with triglycerid(TG),total cholesterol(TC),high density lipoprotein-cholesterol(HDL-C)and low density lipoprotein-cholesterol(LDL-C)levels in this population.Methods In this cmss-sectional study,we recruited 532 Hazakh subjects(30～60 years old)from the pastoral areas in Xinjiang.Peripheral blood was collected fronl all recruited subjects.The DNA was extracted by a standard phenolchloroform method.Then the genotypes of β2-AR was identified by PCR-restriction fragment length polymorphism in all subjects,and the relationships between the polymorphism with the levels of TG,TC,HDL-C and LDL-C were studied.Results In this population,the frequencies of the genotype CC,CT were 98.86% and 1.14%,respectively,no TT was found.The genotype and allele frequencies were in Hardy-Weinberg equilibrium(x2=0.017,P=0.896).The levels of TG,TC,HDL,LDL were increased in CT,but no significant difference was found between these two genotypes in this population.The TC genotype had higher LDL-C level than TT genotvpe in female,and the difference was significant(P=0.038),but according to the standard announced by WHO,genotype CT and T allele were associated with high LDL-C(P=0.031). Conclusion These data sugges that the+491C＞T genotype of β2-AR gene is associated with higher LDL-C level in this population,especially in female,which sugges that it may play an imporlant role in abnormal LDL-C in fenmle Hazakh of Xinjiang. Key words: β2-adrenergic receptor gene;  +491C＞T polymorphism; Hazakh; Lipid