Additional file 8: of A de novo variant in ADGRL2 suggests a novel mechanism underlying the previously undescribed association of extreme microcephaly with severely reduced sulcation and rhombencephalosynapsis

Figure S5. Cytoskeletal organization is altered in HeLa cells overexpressing mutant ADGRL2. Seventy two hours after transfection, HeLa cells were processed for histochemistry using phalloidin conjugates for F-actin labelling (red), alpha-tubulin antibody (green) and Hœscht as a nucleic acid stain (blue). a HeLa cells overexpressing the pcD-empty plasmid present predominant fusiform shapes with few focal contacts. b HeLa cells overexpressing Wt pcDCIRL-2 are characterized by spread out cytoplasms with numerous focal contacts (arrows). c HeLa cells overexpressing Mt. pcDCIRL-2 present very large and spread out cytoplasms with a very high density of focal contacts (arrows). (TIF 7878 kb)