Investigation of Mechanisms of Interaction of Yersinia pestis EV NIIEG Vaccine Strain Bacteria with Human Red Blood Cells

The comparative experiments demonstrated strong correlation between the index of Yersinia pestis EV NIIEG strain adhesion to human red blood cells and hydrophobic properties of bacteria. At the same time no dependence between hemagglutination and adhesion of plague microbe was determined. Bacterial culture treatment with trypsin and high temperature (56 °C) decreased adhesion. Incubation of microbial cells with red blood cells in the presence of antibiotics (ampicillin, gentamycin, doxycycline and cefotaxime) had no influence on their interaction. The results of investigations suggested the major role of hydrophobic interactions in Y.pestis EV NIIEG strain adhesion to human red blood cells provided by the surface structures of microbial cells of the lipoprotein origin.