Comprehensive metabolic modeling of multiple 13C-isotopomer data sets to study metabolism in perfused working hearts

The present study is the first to provide comprehensive metabolic modeling for the perfused working heart using 13C-metabolic flux analysis. Our approach integrates 12 data sets (with 4 unique 13C-tracers) into a single metabolic model, identifies inconsistencies in measurement data, and improves relative flux precision compared with previous algebraic models.