Особенности дегрануляции нейтрофильных гранулоцитов под действием различных стимуляторов

We studied an action of the immunomodulator Glutoxim, lipopolysaccharide and N-formyl-methionyl-leucyl-phenylalanine on the degranulation of neutrophils, both isolated and in the composition of whole blood. It was established that Glutoxim stimulates the secretion of peptides and proteins from the granules of neutrophils. Glutoxim as well as lipopolysaccharide and N-formyl-methionyl-leucyl-phenylalanine demonstrate higher degranulation activity for neutrophils in whole blood, than for isolated neutrophils. It is shown that a longer incubation time of whole blood with Glutoxim and lipopolysaccharide is associated with decreased concentration of neutrophil peptides and proteins in the plasma, which may indicate that stimulation by Glutoxim and lipopolysaccharide, unlike stimulation by N-formyl-methionyl-leucyl-phenylalanine, can lead to activating of the receptors of blood cells and binding of secreted compounds by the cells. Secretory activity Glutoxim depends on phosphodiesterases, since this activity can be blocked by the addition of the phosphodiesterases inhibitor pentoxifylline.