Microfluidics for monitoring and imaging pancreatic islet and β-cells for human transplant

Abstract: This chapter discusses various microfluidic technologies that have been developed to study islets and β-cells. The chapter first introduces key issues in the field of pancreatic islet transplantation as a clinical therapy for Type I diabetes. It then reviews microfluidic technologies that have been developed for the study of pancreatic islet and β-cell physiology and disease pathophysiology. The chapter then describes the design, fabrication, and application of UlC’s microfluidic-based multimodal islet perifusion and live-cell imaging system. Protocols are available at the end of the chapter.