Antimicrobial Activity of Fusidic Acid Inclusion Complexes.

ABSTRACT Objectives To synthesize and characterize the inclusion complexes of fusidic acid with β – cyclodextrin, followed by the evaluation of their antimicrobial activity against pure stain (staphylococcus aureus ATCC 25923) and against isolated staphylococcus from clinical cases. Methods The desired compounds were synthesized using a molar ratio of fusidic acid: β-cyclodextrin of 1:1. Synthesized compounds were analyzed by Fourier transform Infrared Spectroscopy (FTIR), XRay Diffraction (XRD), Scanning Electron Microscopy (SEM) and also Thermal Analysis and the results confirmed the formation of inclusion compounds by fusidic acid with β-cyclodextrin. Results Physical – chemical characterization confirmed the preparation of desired inclusion compounds, and the antimicrobial test confirmed that all compounds obtained have antimicrobial activity. Antimicrobial activity of freeze drying complex against Staphilococcus aureus is similar with pure fusidic acid activity, being better than cefoxitin one. Similar behavior being observed against methicillin resistant sthapylococcus aureus, and sthaphilococcus epidermidis. Conclusions In present work three different inclusion complexes of fusidic acid were prepared by using three different preparation methods. All inclusion complexes obtained represented good antimicrobial activity against different staphylococcus aureus stains. Antimicrobial activity of these new prepared compounds was observed to be better than the one of cefoxitin.