Method for simultaneously improving specific activity and heat stability of transglutaminase

The invention discloses a method for simultaneously improving specific activity and heat stability of transglutaminase, and belongs to the field of enzyme engineering. Mutant enzyme with improved specific activity and heat stability at the temperature of 50 DEG C is obtained by using the site-specific mutagenesis technology to insert an amino acid sequence, coming from the vicinity of an enzyme activity center, between streptomyces hygroscopicus transglutaminase leading peptide and maturase. The method is more suitable for industrial production and application.