Effect of glyprolines PGP and N-acetyl-PGP on the expression of cytokine genes during stress- and acetate-induced ulceration

We have studied the effects of glyprolines PGP and N-acetyl-PGP on cytokine gene expression during stress and acetate-induced ulceration. Levels of RNA in mononuclear cells in circulating blood were evaluated using reverse transcription polymerase chain reaction. Intranasal administration of PGP (3.7 μmol/kg) has had a significant protective effect against stress-induced (59.4%) and acetate-induced (78.5%) ulceration in rats. N-acetyl-PGP has not changed the area of stress-induced damage, but it tended to inhibit the development of acetate ulcers. We found that stress-induced damage of gastric mucosa is accompanied by an increase in TNFα transcription and a decrease in IL-4 transcription. The development of acetate ulcers is accompanied by a decrease in expression of a number of cytokines: IFNα, IFNγ, IL-2, IL-4, IL-6, IL-12, and TNFα. The protective effect of PGP is accompanied by an increase in expression of IL-6. When N-acetyl-PGP is administered, an increase in the expression of Th-1 and Th-2 cytokines have been observed: IFNα, IFNγ, and IL-4.