The progestin levonorgestrel affects hypothalamus–pituitary–gonad axis in pubertal roach (Rutilus rutilus)

s / Toxicology Letters 229S (2014) S40–S252 S115 pubertal roach by affecting the pituitary gonadotropin expression and the sex steroid levels. This disruption was determined to occur in males after exposure to an environmentally relevant concentration (31ng/L LNG). Interestingly, the highest tested concentration of LNG (3124ng/L) exerted an oestrogenic effect on fish of both sexes (as indicatedbyupregulated levels of vitellogenin andoestrogen receptor 1 mRNA in the liver of exposed fish). This study was supported by the Project of the Czech Science Foundation (No. P503/12/P165), the CENAKVA project (CZ.1.05/2.1.00/01.0024), and the project LO1205 with a financial support from the MEYS of the CR under the NPU I program. R. G. was also supported in part by CZ.1.07/2.3.00/20.0024. http://dx.doi.org/10.1016/j.toxlet.2014.06.413