Analysis of heat shock protein 70 gene polymorphisms in Fujian Han population of China

Objective To understand the distribution of heat shock protein 70(HSP70) genepolymorphisms in Chinese Han population and to provide the genetic background for the co-relation between HSP70 polymorphism and disease.Methods Three polymorphic loci in HSP70 gene in 127 normal Chinese Han people in Fujian province were analysed by PCR and restriction enzyme analysis and compared the genotype and allele frequency of HSP70 in different populations from different area.Results HSP70-1 genotype GG,GC and CC are 55.1%,40.2% and 4.7% respectively,HSP70-2 genotype AA,AG and GG are 44.1%,48.8% and 6.9% respectively,and HSP70-hom genotype TT,TC and CC are 59.8%,37.0% and 3.2% respectively.The allele frequencies of G and C in HSP70-1 are 75.2% and 24.8% respectively.The allele frequencies of G and A in HSP70-2 are 68.5% and 31.5% respectively.The allele frequencies of T and C in HSP70-hom are 78.3% and 21.7% respectively.The distribution of HSP70-1 polymorphism in Chinese Han population is almost the same as it is in Japanese and Mexican populations,but different from that in American and Spanish populations,the difference is significant(P0.05).The frequency of GG homozygote in HSP70-1 is significantly higher than that in American and Spanish populations.No significant difference was found in the distribution of HSP70-2 polymorphism between Chinese and Japanese,but different from that in Mexican,American and Spanish populations,the difference is significant(P0.05).The frequency of AA homozygote in HSP70-2 is significantly higher than that in Mexican,American and Spanish populations.The distribution of HSP70-hom genotype and allele frequency in Chinese Han population is almost the same as it is in Japanese and Mexican populations.The genotype distribution and allele frequency of HSP70 in Fujian Han population is almost the same as those in Taiwan Han population, but is different from those in Wuhan Han population in certain loci.Conclusion The distribution of HSP70 gene polymorphism in Chinese Han population is different from that in some other regions.The difference exists as regard to race and region.