Characterization of Elastolytic Protease, Asnilase from Aspergillus nidulans and Its Cytotoxicity on Human Endothelial and Epithelial Cells

Aims: To elucidate the pathogenicity of Aspergillus nidulans , elastolytic protease was isolated from the culture supernatant of the isolate, and its biological activity and cytotoxicity were examined. Methodology: A. nidulans (NBRC 4340) spores were cultured on YCB-elastin medium for three days and elastolytic protease was isolated from the culture supernatant by DEAE-Cellulose anion exchange chromatography. Elastolytic activity was measured by using GAAPLNA (Glutaryl-L-alanylOriginal Research Article