A study on the sterility, safety, potency and purity of hydropericardium hepatitis syndrome (Angara disease) vaccines

The present study was conducted in order to determine the quality of commercially available Angara disease vaccines. For the experiment, 200 broiler chicks of one day old were reared at National Veterinary Laboratories, Islamabad in isolation units. Chicks were divided into three groups that is, 1, 2, 3 and having 25, 25 and 150 chicks, respectively. Group 1 was used to determine LD50 of the virus in chicks, whereas the chicks of group 2 and 3 were used to assess the safety and potency of the vaccines. The LD50 (lethal dose 50) of the virus was determined in 26 days old broiler chickens divided into sub-groups a, b, c, d and e. The LD50 titre of the (10%) viral suspension was prepared from liver